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TJAP1 Monoclonal antibody

TJAP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1F10

Cat no : 68495-1-Ig

Print datasheet

Synonyms

DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4

验证数据展示

  • WB analysis using 68495-1-Ig

    WB analysis using 68495-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HepG2 using 68495-1-Ig

    WB analysis of HepG2 using 68495-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, HSC-T6 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68495-1-Ig targets TJAP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen TJAP1 fusion protein Ag11807 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tight junction associated protein 1 (peripheral)
Synonyms DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4
Calculated Molecular Weight 547 aa, 61 kDa
Observed Molecular Weight 86-95 kDa
GenBank Accession NumberBC064401
Gene Symbol TJAP1
Gene ID (NCBI) 93643
RRIDAB_3085206
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ5JTD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

实验方案

Product Specific Protocols
WB protocol for TJAP1 antibody 68495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols