验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13819-1-AP targets TKTL1 in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | TKTL1 fusion protein Ag4913 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transketolase-like 1 |
Synonyms | TK 2, TKR, TKT2, TKTL1, Transketolase 2, transketolase like 1, Transketolase like protein 1, Transketolase related protein |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC025382 |
Gene Symbol | TKTL1 |
Gene ID (NCBI) | 8277 |
RRID | AB_3085424 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51854 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transketolase (TKT) is an enzyme that is ubiquitously expressed in all living organisms and has been identified as an important regulator of cancer. The TKT family includes the TKT gene and two TKT-like (TKTL) genes; TKTL1 and TKTL2.The basic components ribose-5-phosphate and acetyl-CoA, which are formed by TKTL1, provide essential building blocks for the formation of new cells. TKTL1 also allows survival in the absence of oxygen (hypoxia).TKTL1 also has been shown to be involved in regulating neuronal development in the cerebral cortex.
实验方案
Product Specific Protocols | |
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WB protocol for TKTL1 antibody 13819-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |