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验证数据展示

  • WB analysis using 13819-1-AP

    WB analysis using 13819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13819-1-AP (TKTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TKTL1 Polyclonal antibody

TKTL1 Polyclonal Antibody for WB, ELISA
Cat No. 13819-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TK 2, TKR, TKT2, TKTL1, Transketolase 2, transketolase like 1, Transketolase like protein 1, Transketolase related protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13819-1-AP targets TKTL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TKTL1 fusion protein Ag4913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transketolase-like 1
Synonyms TK 2, TKR, TKT2, TKTL1, Transketolase 2, transketolase like 1, Transketolase like protein 1, Transketolase related protein
Calculated Molecular Weight 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC025382
Gene Symbol TKTL1
Gene ID (NCBI) 8277
RRIDAB_3085424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transketolase (TKT) is an enzyme that is ubiquitously expressed in all living organisms and has been identified as an important regulator of cancer. The TKT family includes the TKT gene and two TKT-like (TKTL) genes; TKTL1 and TKTL2.The basic components ribose-5-phosphate and acetyl-CoA, which are formed by TKTL1, provide essential building blocks for the formation of new cells. TKTL1 also allows survival in the absence of oxygen (hypoxia).TKTL1 also has been shown to be involved in regulating neuronal development in the cerebral cortex.

实验方案

Product Specific Protocols
WB protocol for TKTL1 antibody 13819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols