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  • Featured Product
  • KD/KO Validated

TLE1 Monoclonal antibody, PBS Only

TLE1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F2H11

Cat no : 66216-1-PBS

Print datasheet

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

验证数据展示

  • WB analysis of HEK-293 using 66216-1-Ig (same clone as 66216-1-PBS)

    WB analysis of HEK-293 using 66216-1-Ig (same clone as 66216-1-PBS)

    WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66216-1-Ig (same clone as 66216-1-PBS)

    WB analysis of Jurkat using 66216-1-Ig (same clone as 66216-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

产品信息

66216-1-PBS targets TLE1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TLE1 fusion protein Ag21327 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Synonyms E(Sp1) homolog, ESG, ESG1, GRG1, TLE1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04724
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).