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验证数据展示

  • WB analysis using 30959-1-AP

    WB analysis using 30959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30959-1-AP (TLE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 30959-1-AP

    IP experiment of K-562 using 30959-1-AP

    IP result of anti-TLE4 (IP:30959-1-AP, 4ug; Detection:30959-1-AP 1:800) with K-562 cells lysate 1720 ug.

TLE4 Polyclonal antibody

TLE4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30959-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

GRG4, KIAA1261

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Neuro-2a cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30959-1-AP targets TLE4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 TLE4 fusion protein Ag34006 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
别名 GRG4, KIAA1261
计算分子量 84 kDa
观测分子量76-88 kDa
GenBank蛋白编号BC059405
基因名称 TLE4
Gene ID (NCBI) 7091
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ04727
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE4 (Transducin-like enhancer protein 4), also known as GRG4. It is predicted to be located in the nucleus. Tle4 promotes acquisition of corticothalamic identity and blocks emergence of core characteristics of subcerebral/corticospinal projection neuron identity, including gene expression and connectivity. During the first post-natal week, when corticothalamic innervation is ongoing, Tle4 is required to stabilize corticothalamic neuron identity, limiting interference from differentiation programs of developmentally related neuron classes (PMID: 37561632). The molecular weight of TLE4 is 76 kDa.

实验方案

Product Specific Protocols
WB protocol for TLE4 antibody 30959-1-APDownload protocol
IP protocol for TLE4 antibody 30959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols