验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
13979-1-AP targets TLK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TLK2 fusion protein Ag5022 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tousled-like kinase 2 |
| 别名 | TLK 2, Serine/threonine-protein kinase tousled-like 2, PKU-alpha, PKU ALPHA, HsHPK |
| 计算分子量 | 772 aa, 88 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC044925 |
| 基因名称 | TLK2 |
| Gene ID (NCBI) | 11011 |
| RRID | AB_2203887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86UE8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TLK2 antibody 13979-1-AP | Download protocol |
| IHC protocol for TLK2 antibody 13979-1-AP | Download protocol |
| IF protocol for TLK2 antibody 13979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Gene Ther Targeting TLK2 inhibits the progression of gastric cancer by reprogramming amino acid metabolism through the mTOR/ASNS axis
| ||
Front Genet Overexpression of tousled-like kinase 2 predicts poor prognosis in HBV-related hepatocellular carcinoma patients after radical resection | ||
Transl Cancer Res TLK2 promotes progression of hepatocellular carcinoma through Wnt/β-catenin signaling |
