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Anti-Human TLR3/CD283 Rabbit Recombinant Antibody

TLR3/CD283 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230445G9

Cat no : 98016-2-RR

Print datasheet

Synonyms

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445G9

验证数据展示

  • FC experiment of human monocyte-derived immature dendritic cells using 98016-2-RR

    FC experiment of human monocyte-derived immature dendritic cells using 98016-2-RR

    1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-2-RR, Clone: 230445G9) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD11c. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human monocyte-derived immature dendritic cells using 98016-2-RR

    FC experiment of human monocyte-derived immature dendritic cells using 98016-2-RR

    1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-2-RR, Clone: 230445G9) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD11c. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98016-2-RR

    Affinity and Kinetic Characterization of 98016-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98016-2-RR against Human TLR3/CD283 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inhuman monocyte-derived immature dendritic cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98016-2-RR is TLR3/CD283 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen TLR3/CD283 fusion protein Ag34044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 3
Synonyms TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445G9
Calculated Molecular Weight 904 aa, 104 kDa
GenBank Accession NumberBC094737
Gene Symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15455
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).

实验方案

Product Specific Protocols
FC protocol for TLR3/CD283 antibody 98016-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols