验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | human monocyte-derived immature dendritic cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-98016-2 targets TLR3/CD283 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | TLR3/CD283 fusion protein Ag34044 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | toll-like receptor 3 |
| 别名 | TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445G9 |
| 计算分子量 | 904 aa, 104 kDa |
| GenBank蛋白编号 | BC094737 |
| 基因名称 | TLR3 |
| Gene ID (NCBI) | 7098 |
| RRID | AB_3673785 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15455 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 TLR3/CD283 antibody CL750-98016-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |