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验证数据展示

  • WB analysis of human lung using 12033-1-AP

    WB analysis of human lung using 12033-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12033-1-AP

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12033-1-AP

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 12033-1-AP

    IF Staining of PC-3 using 12033-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using TM7SF2 antibody (12033-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TM7SF2 Polyclonal antibody

TM7SF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12033-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman lung tissue, BxPC-3 cells, human heart tissue, human kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12033-1-AP targets TM7SF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 TM7SF2 fusion protein Ag2637 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane 7 superfamily member 2
别名 C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta
计算分子量 418 aa, 46 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC012857
基因名称 TM7SF2
Gene ID (NCBI) 7108
RRIDAB_2203934
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76062
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TM7SF2 antibody 12033-1-APDownload protocol
IHC protocol for TM7SF2 antibody 12033-1-APDownload protocol
IF protocol for TM7SF2 antibody 12033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.

Authors - Monika Lewinska
View Article