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TM7SF2 Polyclonal antibody

TM7SF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 12033-1-AP

Print datasheet

Synonyms

C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta

验证数据展示

  • WB analysis of human lung using 12033-1-AP

    WB analysis of human lung using 12033-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12033-1-AP

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12033-1-AP

    IHC staining of human prostate cancer using 12033-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 12033-1-AP

    IF Staining of PC-3 using 12033-1-AP

    Immunofluorescent analysis of PC-3 cells using 12033-1-AP (TM7SF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman lung tissue, BxPC-3 cells, human heart tissue, human kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12033-1-AP targets TM7SF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TM7SF2 fusion protein Ag2637 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane 7 superfamily member 2
Synonyms C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC012857
Gene Symbol TM7SF2
Gene ID (NCBI) 7108
RRIDAB_2203934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TM7SF2 antibody 12033-1-APDownload protocol
IHC protocol for TM7SF2 antibody 12033-1-APDownload protocol
IF protocol for TM7SF2 antibody 12033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.

Authors - Monika Lewinska
View Article