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验证数据展示

  • WB analysis using 19826-1-AP

    WB analysis using 19826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19826-1-AP (TM7SF3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 19826-1-AP

    WB analysis using 19826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19826-1-AP (TM7SF3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19826-1-AP

    IHC staining of human ovary cancer using 19826-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19826-1-AP (TM7SF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 19826-1-AP

    IF Staining of U-251 using 19826-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TM7SF3 antibody (19826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TM7SF3 Polyclonal antibody

TM7SF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19826-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, HepG2 cells, mouse pancreas tissue, mouse testis tissue, U2OS cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19826-1-AP targets TM7SF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TM7SF3 fusion protein Ag13919 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane 7 superfamily member 3
别名
计算分子量 570 aa, 64 kDa
观测分子量 64-80 kDa
GenBank蛋白编号BC005176
基因名称 TM7SF3
Gene ID (NCBI) 51768
RRIDAB_10638000
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS93
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The seven-transmembrane superfamily member 3 protein (TM7SF3) is a pro-survival factor induced by p53 that regulates protein homeostasis and attenuates cellular stress. TM7SF3 inhibits caspase 3/7 while siRNA-mediated silencing of TM7SF3 accelerates ER stress and the unfolded protein responses (UPR). This involves inhibitory phosphorylation of eIF2α activity and increased expression of ATF3, ATF4, and C/EBP, followed by induction of apoptosis. TM7SF3 maintains cellular reducing power within physiological levels and reduces the content of proapoptotic proteins such as FAS, FADD, and caspase-8. (PMID: 36304109)

实验方案

Product Specific Protocols
WB protocol for TM7SF3 antibody 19826-1-APDownload protocol
IHC protocol for TM7SF3 antibody 19826-1-APDownload protocol
IF protocol for TM7SF3 antibody 19826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols