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TM9SF4 Polyclonal antibody

TM9SF4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29520-1-AP

Print datasheet

Synonyms

dJ836N17.2, KIAA0255, TM9SF4

验证数据展示

  • WB analysis using 29520-1-AP

    WB analysis using 29520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29520-1-AP (TM9SF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 29520-1-AP

    IHC staining of mouse colon using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29520-1-AP

    IHC staining of human stomach cancer using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29520-1-AP

    IHC staining of human stomach cancer using 29520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA375 cells, PC-3 cells, mouse kidney tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29520-1-AP targets TM9SF4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen TM9SF4 fusion protein Ag31253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane 9 superfamily protein member 4
Synonyms dJ836N17.2, KIAA0255, TM9SF4
Calculated Molecular Weight 642 aa, 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC021107
Gene Symbol TM9SF4
Gene ID (NCBI) 9777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TM9SF4 antibody 29520-1-APDownload protocol
IHC protocol for TM9SF4 antibody 29520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols