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TMBIM6 Recombinant antibody

TMBIM6 Uni-rAbTM Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241589D12

Cat no : 84499-1-RR

Print datasheet

Synonyms

TEGT, BI-1, BI1, BI 1, Bax inhibitor 1

验证数据展示

  • IHC staining of mouse liver using 84499-1-RR

    IHC staining of mouse liver using 84499-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84499-1-RR

    IHC staining of mouse liver using 84499-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84499-1-RR (TMBIM6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 84499-1-RR

    IF Staining of mouse liver using 84499-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMBIM6 antibody (84499-1-RR, Clone: 241589D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84499-1-RR

    Affinity and Kinetic Characterization of 84499-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84499-1-RR against Human TMBIM6 were performed. The affinity constant is 6.46 nM.

经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84499-1-RR is TMBIM6 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TMBIM6 fusion protein Ag24780 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane BAX inhibitor motif containing 6
Synonyms TEGT, BI-1, BI1, BI 1, Bax inhibitor 1
Calculated Molecular Weight 27 kDa
GenBank Accession NumberBC000916
Gene Symbol TMBIM6
Gene ID (NCBI) 7009
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMBIM6 antibody 84499-1-RRDownload protocol
IF protocol for TMBIM6 antibody 84499-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols