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验证数据展示

  • WB analysis using 30393-1-AP

    WB analysis using 30393-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30393-1-AP (TMED5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

TMED5 Polyclonal antibody

TMED5 Polyclonal Antibody for WB, ELISA
Cat No. 30393-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Transmembrane emp24 domain-containing protein 5, p28, p24gamma2, p24 family protein gamma-2, CGI-100

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MKN-45 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30393-1-AP targets TMED5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMED5 fusion protein Ag33253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane emp24 protein transport domain containing 5
别名 Transmembrane emp24 domain-containing protein 5, p28, p24gamma2, p24 family protein gamma-2, CGI-100
观测分子量25 kDa
GenBank蛋白编号BC070051
基因名称 TMED5
Gene ID (NCBI) 50999
RRIDAB_3086307
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3A6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMED5 ( transmembrane p24 trafficking protein 5) is highly expressed in cervical and bladder cancer cell lines. TMED5 promotes nuclear autophagy and the malignant behavior of cervical cancer cells (PMID:36530030). Moreover, TMED5 could interact with WNT7B and thus activate the canonical WNT-CTNNB1/β-catenin signaling pathway (PMID:30394198).

实验方案

Product Specific Protocols
WB protocol for TMED5 antibody 30393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols