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TMED9 Polyclonal antibody
TMED9 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21620-1-AP targets TMED9 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Immunogen | TMED9 fusion protein Ag13813 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transmembrane emp24 protein transport domain containing 9 |
Synonyms | Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9 |
Calculated Molecular Weight | 235 aa, 27 kDa |
Observed Molecular Weight | 24-27 kDa |
GenBank Accession Number | BC001123 |
Gene Symbol | TMED9 |
Gene ID (NCBI) | 54732 |
RRID | AB_10858623 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BVK6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TMED9 antibody 21620-1-AP | Download protocol |
IHC protocol for TMED9 antibody 21620-1-AP | Download protocol |
IF protocol for TMED9 antibody 21620-1-AP | Download protocol |
IP protocol for TMED9 antibody 21620-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. | ||
Cell A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
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Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
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BMC Biol ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier. | ||
Bioengineered High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.
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Adv Sci (Weinh) TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. |