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验证数据展示

  • WB analysis using 11928-1-AP

    WB analysis using 11928-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included
  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included

TMEFF2 Polyclonal antibody

TMEFF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11928-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TMEFF2 Ready-To-Use IHC Kit. TMEFF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 TMEFF2 fusion protein Ag2609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein with EGF-like and two follistatin-like domains 2
别名 HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2
计算分子量 374 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC008973
基因名称 TMEFF2
Gene ID (NCBI) 23671
RRIDAB_2204477
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEFF2 antibody 11928-1-APDownload protocol
IHC protocol for TMEFF2 antibody 11928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.

Authors - Condomines Maud M
View Article
humanIHC

Cancer Cell Int

TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.

Authors - Sidi Xie
View Article