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TMEFF2 Polyclonal antibody

TMEFF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11928-1-AP

Print datasheet

Synonyms

HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2

验证数据展示

  • WB analysis using 11928-1-AP

    WB analysis using 11928-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included
  • IHC staining of human prostate cancer using 11928-1-AP

    IHC staining of human prostate cancer using 11928-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEFF2 antibody included

经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TMEFF2 Ready-To-Use IHC Kit. TMEFF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen TMEFF2 fusion protein Ag2609 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein with EGF-like and two follistatin-like domains 2
Synonyms HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2
Calculated Molecular Weight 374 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC008973
Gene Symbol TMEFF2
Gene ID (NCBI) 23671
RRIDAB_2204477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEFF2 antibody 11928-1-APDownload protocol
IHC protocol for TMEFF2 antibody 11928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.

Authors - Condomines Maud M
View Article
humanIHC

Cancer Cell Int

TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.

Authors - Sidi Xie
View Article