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  • Featured Product
  • KD/KO Validated

TMEM106B (122-140aa) Polyclonal antibody

TMEM106B (122-140aa) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30301-1-AP

Print datasheet

Synonyms

FLJ11273, TMEM106B, transmembrane protein 106B

验证数据展示

  • WB analysis using 30301-1-AP

    WB analysis using 30301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30301-1-AP (TMEM106B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30301-1-AP

    WB analysis of HeLa using 30301-1-AP

    WB result of TMEM106B antibody (30301-1-AP; 1:600; room temperature for 1.5 hours) with negative control and TMEM106B knockout HeLa cells.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30301-1-AP targets TMEM106B (122-140aa) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 106B
Synonyms FLJ11273, TMEM106B, transmembrane protein 106B
Calculated Molecular Weight 31 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC033901
Gene Symbol TMEM106B
Gene ID (NCBI) 54664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-55 kDa and 70-90 kDa (Glycosylated or Dimer) form in western blot test. (PMID: 27543298, 22895706, PMID: 23136129).

实验方案

Product Specific Protocols
WB protocol for TMEM106B (122-140aa) antibody 30301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols