• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

TMEM111 Polyclonal antibody

TMEM111 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24645-1-AP

Print datasheet

Synonyms

EMC3, POB

验证数据展示

  • WB analysis using 24645-1-AP

    WB analysis using 24645-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24645-1-AP (TMEM111 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 24645-1-AP

    WB analysis of HEK-293 using 24645-1-AP

    WB result of TMEM111 antibody (24645-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells.

  • IF Staining of A431 using 24645-1-AP

    IF Staining of A431 using 24645-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (24645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 24645-1-AP

    IHC staining of human ovary cancer using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24645-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24645-1-AP (TMEM111 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HuH-7 cells, MDA-MB-453s cells, NIH/3T3 cells, mouse brain tissue, mouse stomach tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24645-1-AP targets TMEM111 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM111 fusion protein Ag18236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 111
Synonyms EMC3, POB
Calculated Molecular Weight 261 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC022807
Gene Symbol TMEM111
Gene ID (NCBI) 55831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0I2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM111 antibody 24645-1-APDownload protocol
IHC protocol for TMEM111 antibody 24645-1-APDownload protocol
IF protocol for TMEM111 antibody 24645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols