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验证数据展示

  • WB analysis using 83448-6-RR

    WB analysis using 83448-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83448-6-RR (TMEM119 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83448-6-RR

    Affinity and Kinetic Characterization of 83448-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83448-6-RR against Human TMEM119 were performed. The affinity constant is 9.33 nM.

TMEM119 Recombinant antibody

TMEM119 Recombinant Antibody for WB, ELISA
Cat No. 83448-6-RR

产品说明书

CloneNo. 240433F2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83448-6-RR targets TMEM119 in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 119
别名 UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2
计算分子量29 kDa
观测分子量52 kDa
GenBank蛋白编号NM_181724.2
基因名称 TMEM119
Gene ID (NCBI) 338773
RRIDAB_3671086
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ4V9L6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for TMEM119 antibody 83448-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols