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验证数据展示

  • WB analysis using 83448-6-RR

    WB analysis using 83448-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83448-6-RR (TMEM119 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83448-6-RR

    Affinity and Kinetic Characterization of 83448-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83448-6-RR against Human TMEM119 were performed. The affinity constant is 9.33 nM.

TMEM119 Recombinant antibody

TMEM119 Recombinant Antibody for WB, ELISA
Cat No. 83448-6-RR

Print datasheet

CloneNo. 240433F2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83448-6-RR targets TMEM119 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 119
Synonyms UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2
Calculated Molecular Weight29 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberNM_181724.2
Gene Symbol TMEM119
Gene ID (NCBI) 338773
RRIDAB_3671086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ4V9L6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for TMEM119 antibody 83448-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols