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TMEM132E Recombinant antibody

TMEM132E Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230525E12

Cat no : 82983-2-RR

Print datasheet

Synonyms

TMEM132E, transmembrane protein 132E

验证数据展示

  • WB analysis using 82983-2-RR

    WB analysis using 82983-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 82983-2-RR (TMEM132E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82983-2-RR

    Affinity and Kinetic Characterization of 82983-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82983-2-RR against Human TMEM132E were performed. The affinity constant is 0.254 nM.

经过测试的应用

Positive WB detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82983-2-RR targets TMEM132E in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TMEM132E fusion protein Ag26772 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 132E
Synonyms TMEM132E, transmembrane protein 132E
Calculated Molecular Weight 116 kDa
Observed Molecular Weight116 kDa
GenBank Accession NumberNM_001304438
Gene Symbol TMEM132E
Gene ID (NCBI) 124842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6IEE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 132E (TMEM132E) is a member of the TMEM132 family which also include TMEM132A, TMEM132B, TMEM132C, TMEM132D (PMID: 29088312). The gene of TMEM132E is located within the DFNB99 locus on chromosome 17q12 and is highly expressed in the inner ear and other tissues including brain, kidney, lung, liver, spleen, heart, small intestine, colon, thymus, and stomach (PMID: 31656313). A variant of TMEM132E has been associated with autosomal-recessive nonsyndromic hearing loss DFNB99 (PMID: 25331638).

实验方案

Product Specific Protocols
WB protocol for TMEM132E antibody 82983-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols