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TMEM139 Polyclonal antibody

TMEM139 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21912-1-AP

Print datasheet

Synonyms

TMEM139, transmembrane protein 139

验证数据展示

  • WB analysis of mouse lung using 21912-1-AP

    WB analysis of mouse lung using 21912-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21912-1-AP (TMEM139 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21912-1-AP

    IHC staining of human lung cancer using 21912-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21912-1-AP (TMEM139 Antibody) at dilution of 1:50.

  • IHC staining of human lung cancer using 21912-1-AP

    IHC staining of human lung cancer using 21912-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21912-1-AP (TMEM139 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21912-1-AP is TMEM139 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TMEM139 fusion protein Ag15354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 139
Synonyms TMEM139, transmembrane protein 139
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight 24-30 kDa
GenBank Accession NumberBC035517
Gene Symbol TMEM139
Gene ID (NCBI) 135932
RRIDAB_2878945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM139 antibody 21912-1-APDownload protocol
IHC protocol for TMEM139 antibody 21912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols