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验证数据展示

  • IHC staining of mouse brain using 24898-1-AP

    IHC staining of mouse brain using 24898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24898-1-AP

    IHC staining of mouse brain using 24898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24898-1-AP

    WB analysis using 24898-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMEM161A Polyclonal antibody

TMEM161A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24898-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

UNQ582/PRO1152, Transmembrane protein 161A, AROS-29, AROS 29, Adaptive response to oxidative stress protein 29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24898-1-AP targets TMEM161A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM161A fusion protein Ag18974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 161A
Synonyms UNQ582/PRO1152, Transmembrane protein 161A, AROS-29, AROS 29, Adaptive response to oxidative stress protein 29
Calculated Molecular Weight 479 aa, 54 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC005210
Gene Symbol TMEM161A
Gene ID (NCBI) 54929
RRIDAB_2879785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM161A also named as transmembrane protein 161A is a 479 amino acid protein which belongs to TMEM161 family. The molecular weight of TMEM161A is 56 kDa or 42 kDa. TMEM161A is a multi-pass membrane protein and localizes to the cell membrane. It may be involved in the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and cellular response to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for TMEM161A antibody 24898-1-APDownload protocol
IHC protocol for TMEM161A antibody 24898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols