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验证数据展示

  • IF Staining of HepG2 using 83146-1-RR

    IF Staining of HepG2 using 83146-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83146-1-RR

    FC experiment of HepG2 using 83146-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83146-1-RR

    Affinity and Kinetic Characterization of 83146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

TMEM165 Recombinant antibody

TMEM165 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83146-1-RR

产品说明书

CloneNo. 230517B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Transmembrane protein PT27, Transmembrane protein 165, TPARL, TMPT27, Putative divalent cation/proton antiporter TMEM165

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TMEM165 fusion protein Ag14362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 165
别名 Transmembrane protein PT27, Transmembrane protein 165, TPARL, TMPT27, Putative divalent cation/proton antiporter TMEM165
计算分子量 324 aa, 35 kDa
GenBank蛋白编号BC003545
基因名称 TMEM165
Gene ID (NCBI) 55858
RRIDAB_3670844
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HC07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.

实验方案

Product Specific Protocols
IF protocol for TMEM165 antibody 83146-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols