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TMEM175 Polyclonal antibody

TMEM175 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA and More (3)

Transmembrane protein 175, hTMEM175

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19925-1-AP targets TMEM175 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, monkey
免疫原 TMEM175 fusion protein Ag13890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 175
别名 Transmembrane protein 175, hTMEM175
计算分子量 504 aa, 56 kDa
观测分子量41-45 kDa, 54-60 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
RRIDAB_10666166
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSA9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for TMEM175 antibody 19925-1-APDownload protocol
IHC protocol for TMEM175 antibody 19925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.

Authors - Jinhong Wie
  • KO Validated
humanWB

Cell

TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function.

Authors - Chunlei Cang
humanWB

Ann Neurol

Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies.

Authors - Lynne Krohn
humanWB

Proc Natl Acad Sci U S A

TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.

Authors - Sarah Jinn
  • KO Validated
mouseWB

Mol Med

Modulation of anti-cardiac fibrosis immune responses by changing M2 macrophages into M1 macrophages

Authors - Shiqi Chen
ratWB

Mol Brain

TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion.

Authors - Mengling Zhang
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