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- KD/KO Validated
TMEM175 Polyclonal antibody
TMEM175 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19925-1-AP targets TMEM175 in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IP, CoIP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey |
Immunogen | TMEM175 fusion protein Ag13890 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transmembrane protein 175 |
Synonyms | Transmembrane protein 175, hTMEM175 |
Calculated Molecular Weight | 504 aa, 56 kDa |
Observed Molecular Weight | 41-45 kDa, 54-60 kDa |
GenBank Accession Number | BC005158 |
Gene Symbol | TMEM175 |
Gene ID (NCBI) | 84286 |
RRID | AB_10666166 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BSA9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TMEM175 antibody 19925-1-AP | Download protocol |
IHC protocol for TMEM175 antibody 19925-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.
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Ann Neurol Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies. | ||
Proc Natl Acad Sci U S A TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.
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Mol Brain TMEM175 mediates Lysosomal function and participates in neuronal injury induced by cerebral ischemia-reperfusion. | ||
Hum Mol Genet Functionalization of the TMEM175 p.M393T Variant as a risk factor for Parkinson Disease.
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