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  • Featured Product
  • KD/KO Validated

TMEM175 Monoclonal antibody, PBS Only

TMEM175 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E1

Cat no : 68172-1-PBS

Print datasheet

Synonyms

Transmembrane protein 175, hTMEM175, 1E7E1

验证数据展示

  • WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

    WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

    WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 68172-1-Ig (same clone as 68172-1-PBS)

    IP experiment of mouse brain using 68172-1-Ig (same clone as 68172-1-PBS)

    IP result of anti-TMEM175 (IP:68172-1-Ig, 4ug; Detection:68172-1-Ig 1:4000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

产品信息

68172-1-PBS targets TMEM175 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen TMEM175 fusion protein Ag13890 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transmembrane protein 175
Synonyms Transmembrane protein 175, hTMEM175, 1E7E1
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC005158
Gene Symbol TMEM175
Gene ID (NCBI) 84286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BSA9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.