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验证数据展示

  • WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

    WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

    WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 68172-1-Ig (same clone as 68172-1-PBS)

    IP experiment of mouse brain using 68172-1-Ig (same clone as 68172-1-PBS)

    IP result of anti-TMEM175 (IP:68172-1-Ig, 4ug; Detection:68172-1-Ig 1:4000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM175 Monoclonal antibody, PBS Only

TMEM175 Monoclonal Antibody for WB, IP, ELISA
Cat No. 68172-1-PBS

产品说明书

CloneNo. 1E7E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IP, ELISA

别名

Transmembrane protein 175, hTMEM175, 1E7E1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68172-1-PBS targets TMEM175 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 TMEM175 fusion protein Ag13890 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 175
别名 Transmembrane protein 175, hTMEM175, 1E7E1
计算分子量 504 aa, 56 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BSA9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.