验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
19825-1-AP targets TMEM176B in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TMEM176B fusion protein Ag13918 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 176B |
| 别名 | LR8, Protein LR8, TMEM176B, transmembrane protein 176B |
| 计算分子量 | 270 aa, 29 kDa |
| 观测分子量 | 31 kDa, 23 kDa |
| GenBank蛋白编号 | BC004358 |
| 基因名称 | TMEM176B |
| Gene ID (NCBI) | 28959 |
| RRID | AB_10638313 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3YBM2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM176B (also known as LR8) is a transmembrane protein belongs to the MS4A family of proteins. It may play a role in the process of maturation of dendritic cells. TMEM176B was first discovered in human lung fibroblasts and is associated with human small cell lung carcinoma. Studies suggest that TMEM176B might be involved in cancer and might serve as targets for antiangiogenic therapy of cancer patients (PMID: 24602018, 22244448). There are two isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM176B antibody 19825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Formulating a TMEM176B blocker in chitosan nanoparticles uncouples its paradoxical roles in innate and adaptive antitumoral immunity | ||
Front Genet Comprehensive Analysis of Expression and Prognostic Value of MS4As in Glioma. | ||
Cancer Cell Targeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation. |
