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TMEM182 Polyclonal antibody

TMEM182 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25366-1-AP

Print datasheet

Synonyms

TMEM182, transmembrane protein 182

验证数据展示

  • WB analysis of mouse skeletal muscle using 25366-1-AP

    WB analysis of mouse skeletal muscle using 25366-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25366-1-AP (TMEM182 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25366-1-AP

    IHC staining of human liver cancer using 25366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 25366-1-AP

    IHC staining of human liver cancer using 25366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25366-1-AP targets TMEM182 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TMEM182 fusion protein Ag21906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 182
Synonyms TMEM182, transmembrane protein 182
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 15 kDa 26 kDa
GenBank Accession NumberBC130308
Gene Symbol TMEM182
Gene ID (NCBI) 130827
RRIDAB_2880046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZP80
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM182 antibody 25366-1-APDownload protocol
IHC protocol for TMEM182 antibody 25366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB Bioadv

TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression

Authors - Hirofumi Morihara
View Article