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TMEM184B Polyclonal antibody

TMEM184B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28031-1-AP

Print datasheet

Synonyms

C22orf5, DKFZp586A1024, DKFZp686F07174, DKFZp686N1150, FM08, HS5O6A, TMEM184B, transmembrane protein 184B

验证数据展示

  • IHC staining of mouse brain using 28031-1-AP

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28031-1-AP

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28031-1-AP targets TMEM184B in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human
Immunogen TMEM184B fusion protein Ag27704 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 184B
Synonyms C22orf5, DKFZp586A1024, DKFZp686F07174, DKFZp686N1150, FM08, HS5O6A, TMEM184B, transmembrane protein 184B
GenBank Accession NumberBC015489
Gene Symbol TMEM184B
Gene ID (NCBI) 25829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 184B (TMEM184B) is a seven-pass transmembrane protein highly expressed in the nervous system and is thought to play a role in neuronal excitability, synaptic structure, and expression of key developmental and adult pathways involved in neuronal differentiation (PMID: 27122027; 37730546). TMEM184B may activate the MAP kinase signaling pathway (PMID: 12761501). TMEM184B has also been reported to be necessary for interleukin-31-induced itch, promoting adult somatosensation through developmental Wnt signaling and promotion of proper pruriceptive gene expression (PMID: 34629389).

实验方案

Product Specific Protocols
IHC protocol for TMEM184B antibody 28031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols