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验证数据展示

  • WB analysis using 28263-1-AP

    WB analysis using 28263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28263-1-AP (TMEM192 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28263-1-AP

    IHC staining of human breast cancer using 28263-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM192 Polyclonal antibody

TMEM192 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28263-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, IHC, ELISA

别名

FLJ38482, TMEM192, transmembrane protein 192

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

28263-1-AP targets TMEM192 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 Human, mouse
文献引用反应性human
免疫原 TMEM192 fusion protein Ag27576 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 192
别名 FLJ38482, TMEM192, transmembrane protein 192
观测分子量 30 kDa
GenBank蛋白编号BC036301
基因名称 TMEM192
Gene ID (NCBI) 201931
RRIDAB_2881099
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY95
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM192 antibody 28263-1-APDownload protocol
IHC protocol for TMEM192 antibody 28263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mol Ther

Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase

Authors - Yang Wang
View Article