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TMEM199 Polyclonal antibody

TMEM199 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25489-1-AP

Print datasheet

Synonyms

C17orf32, TMEM199, transmembrane protein 199

验证数据展示

  • WB analysis of NIH/3T3 using 25489-1-AP

    WB analysis of NIH/3T3 using 25489-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25489-1-AP targets TMEM199 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TMEM199 fusion protein Ag22009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 199
Synonyms C17orf32, TMEM199, transmembrane protein 199
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC033113
Gene Symbol TMEM199
Gene ID (NCBI) 147007
RRIDAB_2880101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).

实验方案

Product Specific Protocols
WB protocol for TMEM199 antibody 25489-1-APDownload protocol
IHC protocol for TMEM199 antibody 25489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2

Authors - Jiming Chen
View Article