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TMEM200C Polyclonal antibody

TMEM200C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28596-1-AP

Print datasheet

Synonyms

TTMA, Transmembrane protein TTMA, Transmembrane protein 200C

验证数据展示

  • WB analysis using 28596-1-AP

    WB analysis using 28596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28596-1-AP (TMEM200C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 28596-1-AP

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28596-1-AP

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28596-1-AP targets TMEM200C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM200C fusion protein Ag29577 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name two transmembrane domain family member A
Synonyms TTMA, Transmembrane protein TTMA, Transmembrane protein 200C
Calculated Molecular Weight 64 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001080209
Gene Symbol TTMA
Gene ID (NCBI) 645369
RRIDAB_3669664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NKL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM200C (Transmembrane protein 200C) may be an integral component of the membrane.

实验方案

Product Specific Protocols
WB protocol for TMEM200C antibody 28596-1-APDownload protocol
IHC protocol for TMEM200C antibody 28596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols