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验证数据展示

  • WB analysis using 28596-1-AP

    WB analysis using 28596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28596-1-AP (TMEM200C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 28596-1-AP

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28596-1-AP

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM200C Polyclonal antibody

TMEM200C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28596-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TTMA, Transmembrane protein TTMA, Transmembrane protein 200C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28596-1-AP targets TMEM200C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM200C fusion protein Ag29577 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 two transmembrane domain family member A
别名 TTMA, Transmembrane protein TTMA, Transmembrane protein 200C
计算分子量 64 kDa
观测分子量70 kDa
GenBank蛋白编号NM_001080209
基因名称 TTMA
Gene ID (NCBI) 645369
RRIDAB_3669664
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6NKL6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM200C (Transmembrane protein 200C) may be an integral component of the membrane.

实验方案

Product Specific Protocols
WB protocol for TMEM200C antibody 28596-1-APDownload protocol
IHC protocol for TMEM200C antibody 28596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols