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TMEM231 Polyclonal antibody

TMEM231 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23731-1-AP

Print datasheet

Synonyms

ALYE870, FLJ22167, hypothetical protein FLJ22167, PRO1886, TMEM231, Transmembrane protein 231

验证数据展示

  • IHC staining of human pancreas using 23731-1-AP

    IHC staining of human pancreas using 23731-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23731-1-AP (TMEM231 Antibody) at dilution of 1:50. (under 10x lens).

  • IHC staining of human pancreas using 23731-1-AP

    IHC staining of human pancreas using 23731-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23731-1-AP (TMEM231 Antibody) at dilution of 1:50. (under 40x lens).

  • IF Staining of MDCK using 23731-1-AP

    IF Staining of MDCK using 23731-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using TMEM231 antibody (23731-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23731-1-AP targets TMEM231 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, canine
Immunogen TMEM231 fusion protein Ag20669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypothetical protein FLJ22167
Synonyms ALYE870, FLJ22167, hypothetical protein FLJ22167, PRO1886, TMEM231, Transmembrane protein 231
Calculated Molecular Weight 345 aa, 39 kDa
GenBank Accession NumberBC010609
Gene Symbol TMEM231
Gene ID (NCBI) 79583
RRIDAB_2879312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6L2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 231 (TMEM231) encodes a 36 kDa two-pass transmembrane protein that is part of the B9 complex and localizes to the base of the ciliary axoneme at the TZ. TMEM231 was first identified as a causative gene for MKS and participates in the formation of the diffusion barrier between the cilia and plasma membrane (PMID: 34912761, 37736303). Tmem231 mutant mice have developmental defects reminiscent of MKS patients, including corticomedullary kidney cysts, polydactyly and hepatic ductal plate malformation (PMID: 25869670).

实验方案

Product Specific Protocols
IHC protocol for TMEM231 antibody 23731-1-APDownload protocol
IF protocol for TMEM231 antibody 23731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols