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TMEM24 Polyclonal antibody

TMEM24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25507-1-AP

Print datasheet

Synonyms

C2CD2 like, C2CD2L, DLNB23, KIAA0285, TMEM24, Transmembrane protein 24

验证数据展示

  • IHC staining of human pancreas cancer using 25507-1-AP

    IHC staining of human pancreas cancer using 25507-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 25507-1-AP

    IHC staining of human pancreas cancer using 25507-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25507-1-AP

    WB analysis of HeLa using 25507-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25507-1-AP (TMEM24 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25507-1-AP targets TMEM24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TMEM24 fusion protein Ag21913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C2CD2-like
Synonyms C2CD2 like, C2CD2L, DLNB23, KIAA0285, TMEM24, Transmembrane protein 24
Calculated Molecular Weight 706 aa, 76 kDa
Observed Molecular Weight 80-100 kDa
GenBank Accession NumberBC022219
Gene Symbol C2CD2L
Gene ID (NCBI) 9854
RRIDAB_2880108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14523
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM24, also named as KIAA0285 and C2CD2L, is identified as a pancreatic islet-enriched protein that plays a key role in regulating glucose-sensitive INS release from the reserve pool of granules. This antibody detects TMEM24 protein with MW 70-100 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM24 antibody 25507-1-APDownload protocol
IHC protocol for TMEM24 antibody 25507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article