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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse pancreas using 19207-1-AP (same clone as 19207-1-PBS)

    WB analysis of mouse pancreas using 19207-1-AP (same clone as 19207-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19207-1-AP (TMEM27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19207-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 19207-1-AP (same clone as 19207-1-PBS)

    IHC staining of human kidney using 19207-1-AP (same clone as 19207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19207-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 19207-1-AP (same clone as 19207-1-PBS)

    IHC staining of human kidney using 19207-1-AP (same clone as 19207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19207-1-AP (TMEM27 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19207-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 19207-1-AP (same clone as 19207-1-PBS)

    IF Staining of HepG2 using 19207-1-AP (same clone as 19207-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using TMEM27 antibody 19207-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19207-1-PBS in a different storage buffer formulation.

TMEM27 Polyclonal antibody, PBS Only

TMEM27 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19207-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

19207-1-PBS targets TMEM27 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TMEM27 fusion protein Ag7668 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 27
别名
计算分子量 25 kDa
观测分子量 32 kDa
GenBank蛋白编号BC050606
基因名称 TMEM27
Gene ID (NCBI) 57393
RRIDAB_10860104
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBJ8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).