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验证数据展示

  • WB analysis of HEK-293 using 24059-1-AP

    WB analysis of HEK-293 using 24059-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24059-1-AP (TMEM27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

TMEM27 Polyclonal antibody

TMEM27 Polyclonal Antibody for WB, ELISA
Cat No. 24059-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Collectrin, NX 17, NX17, TMEM27, transmembrane protein 27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24059-1-AP targets TMEM27 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TMEM27 fusion protein Ag21257 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 27
别名 Collectrin, NX 17, NX17, TMEM27, transmembrane protein 27
计算分子量 25 kDa
观测分子量 23 kDa
GenBank蛋白编号BC050606
基因名称 TMEM27
Gene ID (NCBI) 57393
RRIDAB_2879419
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBJ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).

实验方案

Product Specific Protocols
WB protocol for TMEM27 antibody 24059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols