TMEM27 Recombinant antibody

TMEM27 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82853-2-RR
CloneNo. 240437C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

NX17, NX 17, Collectrin, CLTRN, ACE2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82853-2-RR targets TMEM27 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TMEM27 fusion protein Ag7668 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 27
Synonyms NX17, NX 17, Collectrin, CLTRN, ACE2
Calculated Molecular Weight 25 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC050606
Gene Symbol TMEM27
Gene ID (NCBI) 57393
RRIDAB_3670578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HBJ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM27 (also termed collectrin), a 46 kDa type I transmembrane protein, is a homolog of the noncatalytic domain of angiotensin-converting enzyme-related carboxypeptidase (Ace2). Its expression has only been reported in the brush border membrane of the proximal tubules and collecting ducts of the kidney and in the pancreatic b cell (PMID: 11278314, 16330324). Overexpression of TMEM27 in b cells leads to increased proliferation in vitro and increased pancreatic b cell mass in vivo, and also has been reported to augment glucosestimulated ins secretion (GSIS) (PMID: 16330324, 16330323). The abundance of TMEM27 protein in b cells is furthermore regulated by ectodomain cleavage, which leads to two cleavage products, a 25 kDa N-terminal part that is released into the extracellular space (the shed fragment), and a 22 kDa C-terminal fragment (CTF) remaining in the membrane that is rapidly degraded (PMID: 16330324). TMEM27 is an N-glycosylated protein and can form dimers with MW of 64-75 kDa(patent US 20100119489 A1). TMEM27 can be used as a beta cell mass biomarker (PMID: 20386877, 21907142).

实验方案

Product Specific Protocols
WB protocol for TMEM27 antibody 82853-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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