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验证数据展示

  • WB analysis using 19919-1-AP

    WB analysis using 19919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19919-1-AP (TMEM38B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 19919-1-AP

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 19919-1-AP

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 19919-1-AP

    IF Staining of HepG2 using 19919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM38B antibody (19919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • WB analysis of MCF-7 using 19919-1-AP

    WB analysis of MCF-7 using 19919-1-AP

    WB result of TMEM38B antibody (19919-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM38B transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

TMEM38B Polyclonal antibody

TMEM38B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19919-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Trimeric intracellular cation channel type B, TRICB, TRIC B, D4Ertd89e, C9orf87

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19919-1-AP targets TMEM38B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM38B fusion protein Ag13835 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 38B
别名 Trimeric intracellular cation channel type B, TRICB, TRIC B, D4Ertd89e, C9orf87
计算分子量 291 aa, 33 kDa
观测分子量28 kDa
GenBank蛋白编号BC000049
基因名称 TMEM38B
Gene ID (NCBI) 55151
RRIDAB_2878623
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM38B encodes endoplasmic reticulum membrane monovalent cation-specific channel which is involved in the release of calcium (Ca2+) from intracellular stores. TMEM38B, also known as TRIC-B, is one of the two trimeric intracellular cation (TRIC) channels. TRIC-B deficiency causes bone disease due to defective Ca2+ release and signaling in the bone cells. (PMID: 34036147, PMID: 39385871)

实验方案

Product Specific Protocols
WB protocol for TMEM38B antibody 19919-1-APDownload protocol
IHC protocol for TMEM38B antibody 19919-1-APDownload protocol
IF protocol for TMEM38B antibody 19919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols