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TMEM39A Polyclonal antibody

TMEM39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20430-1-AP

Print datasheet

Synonyms

SUSR2, TMEM39A, transmembrane protein 39A

验证数据展示

  • IF Staining of U2OS using 20430-1-AP

    IF Staining of U2OS using 20430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of human breast cancer using 20430-1-AP

    IHC staining of human breast cancer using 20430-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20430-1-AP (TMEM39A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 20430-1-AP

    IF Staining of U-251 using 20430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis using 20430-1-AP

    WB analysis using 20430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20430-1-AP (TMEM39A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20430-1-AP targets TMEM39A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TMEM39A fusion protein Ag14263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 39A
Synonyms SUSR2, TMEM39A, transmembrane protein 39A
Calculated Molecular Weight 488 aa, 56 kDa
Observed Molecular Weight48-56 kDa
GenBank Accession NumberBC021277
Gene Symbol TMEM39A
Gene ID (NCBI) 55254
RRIDAB_3085605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 39A (TMEM39A, also called SUSR2) encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases (PMID: 31806350).

实验方案

Product Specific Protocols
WB protocol for TMEM39A antibody 20430-1-APDownload protocol
IHC protocol for TMEM39A antibody 20430-1-APDownload protocol
IF protocol for TMEM39A antibody 20430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols