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TMEM45A Polyclonal antibody

TMEM45A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27534-1-AP

Print datasheet

Synonyms

Transmembrane protein 45A, DNAPTP4, DNA polymerase-transactivated protein 4, DERP7, Dermal papilla-derived protein 7

验证数据展示

  • WB analysis using 27534-1-AP

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27534-1-AP

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA-253 cells, A431 cells, MDA-MB-231 cells, SKOV-3 cells, HH cells, HFF cells, HaCaT cells, MJ cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27534-1-AP targets TMEM45A in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TMEM45A fusion protein Ag24392 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 45A
Synonyms Transmembrane protein 45A, DNAPTP4, DNA polymerase-transactivated protein 4, DERP7, Dermal papilla-derived protein 7
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC040355
Gene Symbol TMEM45A
Gene ID (NCBI) 55076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM45A, also known as DERP7, DNAPTP4, or FLJ10134, is a transmembrane protein belonging to the TMEM family. TMEM45A is highly expressed in keratinocytes and its expression correlates with keratinization, suggesting a role in normal epidermal physiology(PMID: 22954140; PMID: 24689342). TMEM45A promotes cell proliferation, migration, and invasion in glioblastoma cells, favors epithelial-mesenchymal transition (EMT) in colorectal cancer and promotes cell proliferation by favoring the G1/S cell cycle transition in ovarian cancer cells (PMID: 37936608).

实验方案

Product Specific Protocols
WB protocol for TMEM45A antibody 27534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols