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验证数据展示

  • WB analysis using 27534-1-AP

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27534-1-AP

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TMEM45A Polyclonal antibody

TMEM45A Polyclonal Antibody for WB, ELISA
Cat No. 27534-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Dermal papilla-derived protein 7, DERP7, DNA polymerase-transactivated protein 4, DNAPTP4, Transmembrane protein 45A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA-253 cells, A431 cells, MDA-MB-231 cells, SKOV-3 cells, HH cells, HFF cells, HaCaT cells, MJ cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27534-1-AP targets TMEM45A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TMEM45A fusion protein Ag24392 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 45A
别名 Dermal papilla-derived protein 7, DERP7, DNA polymerase-transactivated protein 4, DNAPTP4, Transmembrane protein 45A
观测分子量70-75 kDa
GenBank蛋白编号BC040355
基因名称 TMEM45A
Gene ID (NCBI) 55076
RRIDAB_3669609
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM45A, also known as DERP7, DNAPTP4, or FLJ10134, is a transmembrane protein belonging to the TMEM family. TMEM45A is highly expressed in keratinocytes and its expression correlates with keratinization, suggesting a role in normal epidermal physiology(PMID: 22954140; PMID: 24689342). TMEM45A promotes cell proliferation, migration, and invasion in glioblastoma cells, favors epithelial-mesenchymal transition (EMT) in colorectal cancer and promotes cell proliferation by favoring the G1/S cell cycle transition in ovarian cancer cells (PMID: 37936608). The predicted molecular weight of TMEM45A is 32 kDa, and 66-98 kDa has been reported in the literature(PMID: 22954140).

实验方案

Product Specific Protocols
WB protocol for TMEM45A antibody 27534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols