验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, A549 cells, HepG2 cells, MCF-7 cells, rat heart tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23992-1-AP targets TMEM55B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | TMEM55B fusion protein Ag21172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 55B |
| 别名 | C14orf9, PtdIns 4,5 P2 4 Ptase I, TMEM55B, transmembrane protein 55B |
| 计算分子量 | 277 aa, 29 kDa |
| 观测分子量 | 29-32 kDa |
| GenBank蛋白编号 | BC002867 |
| 基因名称 | TMEM55B |
| Gene ID (NCBI) | 90809 |
| RRID | AB_2879391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86T03 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM55B is also named as C14orf9 and PIP4P1. TMEM55B is originally identified as phosphatidylinositol-4,5-P24-phosphatases (PtdIns-4,5-P24-phosphatases) that catalyze dephosphorylation of PtdIns-4,5-P2 to PtdIns-5-P. The transmembrane protein TMEM55B is involved in the perinuclear clustering of lysosomes upon starvation. TMEM55B expression is upregulated upon starvation via TFEB and TFE3 activation, and loss of TMEM55B diminishes starvation-induced lysosomal clustering. TMEM55B can bind to the dynein adaptor JIP4, promoting dynein-dynactin-dependent lysosomal trafficking to the perinuclear region. TMEM55B is also suggested to contribute to lysosomal homeostasis and mTORC1 activation via the V-ATPase assembly (PMID: 33537719).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM55B antibody 23992-1-AP | Download protocol |
| IP protocol for TMEM55B antibody 23992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation.
| ||
JCI Insight TFEB-mediated lysosomal exocytosis alleviates high fat diet-induced lipotoxicity in the kidney | ||
Arterioscler Thromb Vasc Biol Phosphatidylinositol-(4,5)-Bisphosphate Regulates Plasma Cholesterol Through LDL (Low-Density Lipoprotein) Receptor Lysosomal Degradation.
| ||
Genes Cells TMEM55B contributes to lysosomal homeostasis and amino acid-induced mTORC1 activation.
| ||
J Cell Biol Tex2 is required for lysosomal functions at TMEM55-dependent ER membrane contact sites
| ||
Nat Commun TMEM55B links autophagy flux, lysosomal repair, and TFE3 activation in response to oxidative stress |
