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验证数据展示

  • WB analysis using 21274-1-AP

    WB analysis using 21274-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21274-1-AP (TMEM64 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of human prostate cancer using 21274-1-AP

    IHC staining of human prostate cancer using 21274-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

TMEM64 Polyclonal antibody

TMEM64 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21274-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein 64

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21274-1-AP targets TMEM64 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM64 fusion protein Ag15753 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 64
Synonyms Transmembrane protein 64
Calculated Molecular Weight 380 aa, 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC113828
Gene Symbol TMEM64
Gene ID (NCBI) 169200
RRIDAB_3085648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YI46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tmem64 (transmembrane protein 64) is a positive modulator of osteoclast differentiation by SERCA2-dependent Ca2+ signaling (PMID: 23395171). Altered Levels of mRNAs for Calcium-Binding/Associated Proteins, Annexin A1, S100A4, and TMEM64 are associated with osteoporosis in peripheral blood mononuclear cells (PMID: 31814858). Tmem64 is involved in the metastatic progression of prostate cancer cells by regulating Wnt3a secretion (PMID: 31289498).

实验方案

Product Specific Protocols
WB protocol for TMEM64 antibody 21274-1-APDownload protocol
IHC protocol for TMEM64 antibody 21274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols