TMEM65 Polyclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

WB result of TMEM65 antibody (21913-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HeLa cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.