• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TMEM65 Recombinant antibody

TMEM65 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230512D7

Cat no : 83219-4-RR

Print datasheet

Synonyms

Transmembrane protein 65, 230512D7

验证数据展示

  • WB analysis using 83219-4-RR

    WB analysis using 83219-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83219-4-RR

    WB analysis using 83219-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83219-4-RR

    Affinity and Kinetic Characterization of 83219-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83219-4-RR against Human TMEM65 were performed. The affinity constant is 0.365 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, PC-3 cells, SH-SY5Y cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83219-4-RR targets TMEM65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM65 fusion protein Ag15509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 65
Synonyms Transmembrane protein 65, 230512D7
Calculated Molecular Weight 240 aa, 26 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC032396
Gene Symbol TMEM65
Gene ID (NCBI) 157378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6PI78
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM65 antibody 83219-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols