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验证数据展示

  • IHC staining of mouse pancreas using 29599-1-AP

    IHC staining of mouse pancreas using 29599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29599-1-AP (TMEM66 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29599-1-AP

    IHC staining of mouse cerebellum using 29599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29599-1-AP (TMEM66 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 29599-1-AP

    IHC staining of mouse pancreas using 29599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29599-1-AP (TMEM66 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM66 Polyclonal antibody

TMEM66 Polyclonal Antibody for IHC, ELISA
Cat No. 29599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

SARAF, FOAP 7, HBV XAg-transactivated protein 3, HBV X-transactivated gene 3 protein, HSPC035

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29599-1-AP targets TMEM66 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TMEM66 fusion protein Ag29388 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 66
别名 SARAF, FOAP 7, HBV XAg-transactivated protein 3, HBV X-transactivated gene 3 protein, HSPC035
计算分子量 339 aa, 37 kDa
GenBank蛋白编号BC015012
基因名称 TMEM66
Gene ID (NCBI) 51669
RRIDAB_3086144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM66/SOCE-associated regulatory factor (SARAF) is an endoplasmic reticulum (ER)-resident transmembrane protein that promotes SOCE inactivation and prevents Ca2+ overfilling of the cell. TMEM66 plays a key role in shaping cytosolic Ca2+ signals and determining the content of the major intracellular Ca2+ stores, which is probably important in protecting the cell from Ca2+ overfilling (PMID: 37007714). TMEM66 is a highly conserved protein in vertebrates. In mammals, TMEM66 is ubiquitously expressed, but has significantly high transcript levels in the immune and neuronal tissues (PMID: 25469256). Moreover, TMEM66 is an androgen-responsive marker for prostate cancer and mTOR-dependent cardiac growth (PMID:32173353).

实验方案

Product Specific Protocols
IHC protocol for TMEM66 antibody 29599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols