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TMEM70 Monoclonal antibody

TMEM70 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C11C3

Cat no : 60195-1-Ig

Print datasheet

Synonyms

TMEM70, transmembrane protein 70

验证数据展示

  • WB analysis of A2780 using 60195-1-Ig

    WB analysis of A2780 using 60195-1-Ig

    A2780 cells were subjected to SDS PAGE followed by western blot with 60195-1-Ig (TMEM70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 60195-1-Ig

    IHC staining of human liver using 60195-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 60195-1-Ig

    IHC staining of human liver using 60195-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 60195-1-Ig

    WB analysis of HeLa using 60195-1-Ig

    WB result of TMEM70 antibody (60195-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM70 transfected HeLa cells.

经过测试的应用

Positive WB detected inA2780 cells, HeLa cells
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60195-1-Ig targets TMEM70 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TMEM70 fusion protein Ag16947 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transmembrane protein 70
Synonyms TMEM70, transmembrane protein 70
Calculated Molecular Weight 260 aa, 29 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC002748
Gene Symbol TMEM70
Gene ID (NCBI) 54968
RRIDAB_10896316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication (PMID:21147908) identified TMEM70 gene defect as a pan-ethnic disorder and further redefined it as the most common cause of nuclear-origin ATP synthase deficiency.

实验方案

Product Specific Protocols
WB protocol for TMEM70 antibody 60195-1-IgDownload protocol
IHC protocol for TMEM70 antibody 60195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mitochondrion

Mitochondrial membrane assembly of TMEM70 protein.

Authors - Hana Kratochvílová
View Article