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  • Featured Product
  • KD/KO Validated

FDX1 Recombinant antibody, PBS Only

FDX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230196B12

Cat no : 82957-1-PBS

Print datasheet

Synonyms

TMEM79, TMEM 79, Mattrin, MATT, 230487H5

验证数据展示

  • WB analysis using 82957-1-RR (same clone as 82957-1-PBS)

    WB analysis using 82957-1-RR (same clone as 82957-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-1-RR (FDX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82957-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82957-1-RR (same clone as 82957-1-PBS)

    WB analysis of HEK-293 using 82957-1-RR (same clone as 82957-1-PBS)

    WB result of FDX1 antibody (82957-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82957-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82957-1-RR (same clone as 82957-1-PBS)

    FC experiment of A431 using 82957-1-RR (same clone as 82957-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human FDX1 (82957-1-RR, Clone:230196B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82957-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82957-1-RR (same clone as 82957-1-PBS)

    IF Staining of HepG2 using 82957-1-RR (same clone as 82957-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-1-RR, Clone: 230196B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82957-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82957-1-RR

    Affinity and Kinetic Characterization of 82957-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82957-1-RR against Human FDX1 were performed. The affinity constant is 4.02 nM.

产品信息

82957-1-PBS targets FDX1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen FDX1 fusion protein Ag3301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ferredoxin 1
Synonyms TMEM79, TMEM 79, Mattrin, MATT, 230487H5
Calculated Molecular Weight 184 aa, 19 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC017063
Gene Symbol FDX1
Gene ID (NCBI) 2230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10109
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.