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TMEM85 Polyclonal antibody

TMEM85 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27708-1-AP

Print datasheet

Synonyms

EMC4, ER membrane protein complex subunit 4, PIG17, TMEM85, transmembrane protein 85

验证数据展示

  • WB analysis using 27708-1-AP

    WB analysis using 27708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27708-1-AP (TMEM85 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

27708-1-AP targets TMEM85 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, rat
Cited Reactivityhuman
Immunogen TMEM85 fusion protein Ag26785 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 85
Synonyms EMC4, ER membrane protein complex subunit 4, PIG17, TMEM85, transmembrane protein 85
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC002583
Gene Symbol TMEM85
Gene ID (NCBI) 51234
RRIDAB_2880950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5J8M3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM85 antibody 27708-1-APDownload protocol
IHC protocol for TMEM85 antibody 27708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
View Article
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
View Article
humanWB

bioRxiv

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

Authors - Katharine R Page
View Article