验证数据展示
经过测试的应用
Positive WB detected in | mouse E12.5 embryos |
Positive IP detected in | Y79 cells |
Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14731-1-AP targets TMEM98 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | TMEM98 fusion protein Ag6492 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transmembrane protein 98 |
别名 | Protein TADA1, TMEM98, transmembrane protein 98 |
计算分子量 | 25 kDa |
观测分子量 | 21-25 kDa |
GenBank蛋白编号 | BC000526 |
基因名称 | TMEM98 |
Gene ID (NCBI) | 26022 |
RRID | AB_11182589 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y2Y6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).
实验方案
Product Specific Protocols | |
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IHC protocol for TMEM98 antibody 14731-1-AP | Download protocol |
IP protocol for TMEM98 antibody 14731-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS Genet The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification. | ||
PLoS Genet Variants in myelin regulatory factor (MYRF) cause autosomal dominant and syndromic nanophthalmos in humans and retinal degeneration in mice. | ||
Aging (Albany NY) The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma. | ||
Invest Ophthalmol Vis Sci Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse.
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