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TMEM9B Polyclonal antibody

TMEM9B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 24331-1-AP

Print datasheet

Synonyms

C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B

验证数据展示

  • WB analysis of rat liver using 24331-1-AP

    WB analysis of rat liver using 24331-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse colon using 24331-1-AP

    WB analysis of mouse colon using 24331-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24331-1-AP

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 24331-1-AP

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inrat liver tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24331-1-AP is TMEM9B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen TMEM9B fusion protein Ag19383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TMEM9 domain family, member B
Synonyms C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight 23 kDa, 35 kDa
GenBank Accession NumberBC040124
Gene Symbol TMEM9B
Gene ID (NCBI) 56674
RRIDAB_2879497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ34
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM9B, also termed as Transmembrane protein 9B encodes a 198 amino-acidprotein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus. There is a dimer form of TMEM9B protein (PMID: 12359240). TMEM9B mRNA is expressed in a wide range of tissues and cells. TMEM9B is a lysosomal transmembrane protein that regulates the activity of inflammatory signaling pathways.

实验方案

Product Specific Protocols
WB protocol for TMEM9B antibody 24331-1-APDownload protocol
IHC protocol for TMEM9B antibody 24331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols