验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, Jurkat cells, mouse colon tissue, rat brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22212-1-AP targets TMF1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TMF1 fusion protein Ag17537 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TATA element modulatory factor 1 |
| 别名 | TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa |
| 计算分子量 | 1093 aa, 123 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC126123 |
| 基因名称 | TMF1 |
| Gene ID (NCBI) | 7110 |
| RRID | AB_2879031 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P82094 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMF1 antibody 22212-1-AP | Download protocol |
| IHC protocol for TMF1 antibody 22212-1-AP | Download protocol |
| IP protocol for TMF1 antibody 22212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
