验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, Daudi cells, HUVEC cells, Raji cells |
| Positive IHC detected in | mouse kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27174-1-AP targets TMIGD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TMIGD1 fusion protein Ag25691 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane and immunoglobulin domain containing 1 |
| 别名 | TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164 |
| 计算分子量 | 262 aa, 29 kDa |
| 观测分子量 | 45 kDa, 29 kDa |
| GenBank蛋白编号 | BC137201 |
| 基因名称 | TMIGD1 |
| Gene ID (NCBI) | 388364 |
| RRID | AB_2880786 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UXZ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMIGD1 antibody 27174-1-AP | Download protocol |
| IHC protocol for TMIGD1 antibody 27174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Intestinal brush border formation requires a TMIGD1-based intermicrovillar adhesion complex | ||
Int Immunopharmacol Macrophage-derived exosomes promote intestinal mucosal barrier dysfunction in inflammatory bowel disease by regulating TMIGD1 via mircroRNA-223 |
