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- KD/KO Validated
TMLHE Polyclonal antibody
TMLHE Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, rat kidney tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
产品信息
16621-1-AP targets TMLHE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit |
| Immunogen | TMLHE fusion protein Ag9919 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | trimethyllysine hydroxylase, epsilon |
| Synonyms | TMLD, TML-alpha-ketoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, EC:1.14.11.8 |
| Calculated Molecular Weight | 376 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC025269 |
| Gene Symbol | TMLHE |
| Gene ID (NCBI) | 55217 |
| RRID | AB_2205303 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVH6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMLHE antibody 16621-1-AP | Download protocol |
| IHC protocol for TMLHE antibody 16621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.
| ||
Cell Rep Inborn Errors of Long-Chain Fatty Acid β-Oxidation Link Neural Stem Cell Self-Renewal to Autism.
| ||
Cell Chem Biol Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite. | ||
bioRxiv αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation |
